We are seeing patients in-person and through Video Visits. Learn more about how we’re keeping you safe and please review our updated visitor policy. Please also consider supporting Weill Cornell Medicine’s efforts to support our front-line workers.


TB Drug Development


A defining interest of our laboratory is the identification of new antibiotic targets and mechanisms. Unlike virtually every other field of medicine, infectious diseases has become progressively less and less effective over time. The reasons for this are multifactorial. However, a commonly overlooked fact is that virtually all antibiotics in clinical use were discovered serendipitously with little foresight. As a result, we lack sufficient knowledge of what defines a good drug target and how to develop new antibiotics from it. We aim to address this deficiency by applying novel mass spectrometry-based metabolomics approaches to gain insight into the underlying biology of Mycobacterium tuberculosis (ie, what makes TB TB) and its response to perturbation at the pharmacologically relevant level of metabolites. While current efforts focus chiefly on Mycobacterium tuberculosis, previous work has included studies of Staphylococcus aureus and Enterococcus faecium.

Ehrt S, Schnappinger D, Rhee KY. Metabolic principles of persistence and pathogenicity in Mycobacterium tuberculosis. Nat Rev Microbiol. 2018 Aug;16(8):496-507.

Jansen RS, Mandyoli L, Hughes R, Wakabayashi S, Pinkham JT, Selbach B, Guinn KM, Rubin EJ, Sacchettini JC, Rhee KY. Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis. Nat Commun. 2020 Apr 23;11(1):1960.

Wang Z, Soni V, Marriner G, Kaneko T, Boshoff HIM, Barry CE 3rd, Rhee KY. Mode-of-action profiling reveals glutamine synthetase as a collateral metabolic vulnerability of M. tuberculosis to bedaquiline. Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19646-19651.

Ballinger E, Mosior J, Hartman T, Burns-Huang K, Gold B, Morris R, Goullieux L, Blanc I, Vaubourgeix J, Lagrange S, Fraisse L, Sans S, Couturier C, Bacqué E, Rhee K, Scarry SM, Aubé J, Yang G, Ouerfelli O, Schnappinger D, Ioerger TR, Engelhart CA, McConnell JA, McAulay K, Fay A, Roubert C, Sacchettini J, Nathan C. Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition. Science. 2019 Feb 1;363(6426):eaau8959.

Isa F, Collins S, Lee MH, Decome D, Dorvil N, Joseph P, Smith L, Salerno S, Wells MT, Fischer S, Bean JM, Pape JW, Johnson WD, Fitzgerald DW, Rhee KY. Mass Spectrometric Identification of Urinary Biomarkers of Pulmonary Tuberculosis. EBioMedicine. 2018 May;31:157-165.

Eoh H, Wang Z, Layre E, Rath P, Morris R, Branch Moody D, Rhee KY. Metabolic anticipation in Mycobacterium tuberculosis. Nat Microbiol. 2017 May 22;2:17084.

Jansen RS, Rhee KY. Emerging Approaches to Tuberculosis Drug Development: At Home in the Metabolome. Trends Pharmacol Sci. 2017 Apr;38(4):393-405.

Contact Information

Infectious Diseases

Roy M. Gulick, M.D., Chief

Avi Bueno, Administrator
Tel: (212) 746-4914
Fax: (212) 746-8675

Patient Appointments & Inquiries

Infectious Diseases Associates & Weill Cornell Travel Medicine
1305 York Avenue, Floor 4
New York, NY 10021
Tel: (646) 962-TRIP (8747)

Division Events

Division News

Division Publications

Clinical Trials

Related Internal Links

PDF icon Division Brochure
PDF icon Travel Clinic Brochure

Related External Links

Center for Special Studies
Clinical & Translational Science Center
Center for Global Health

Patient Care Links

Cornell Clinical Trials Unit
Weill Cornell Travel Medicine

Infectious Diseases Associates